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#31
As long as they are data partitions or drives, you can re letter them no problem. But once you remove the old drive, everything should fall back into place.
If you had a games drive, it`s letter must be the same as it was before you did the clone.
Ideally you want everything to be exactly as it was, which it will be once you remove the old OS drive.
The drive you boot into will always be C and will have the W10 icon on it.