Cannot Boot From USB

Currently, I am using Wiindows 7 Home Premium x86 with the drive in MBR partition style.

I tried to make a bootable USB of latest Windows 10 x86 ISO with Rufus in this configuration:
GPT partition Scheme for UEFI
FAT32
4096 bytes
Quick Format
Create extended label and icon files

When I try to boot from the flash drive, it just goes into a black screen, then returns to the boot menu.

I enabled both capabilities for UEFI and Legacy boot, UEFI is prioritized.

What is the problem? Do I have to reformat the drive first to GPT, or it's not supported? Thank you.

Update:

I tried making a bootable USB with the same configuration in Rufus, except I made it compatible for Legacy BIOS.
I successfully booted the USB. But I want to install Windows 10 in UEFI, any solution?

In the BIOS Settings, booting to both UEFI and Legacy BIOS mode is supported.

I would try letting the Media Creation Tool make the USB flash drive:
Windows 10

You want the blue box near the bottom of the page "Download tool now".

Then try booting that flash drive in UEFI mode.

Some BIOS will try to boot USB in Legacy mode instead of UEFI and fail. To make sure you can press F8 (or whatever it is) to see the boot menu and then manually select the UEFI option for your USB flash drive. This will start with UEFI and your Flash model. If it doesn't start with UEFI it is legacy.

Do you want to boot in UEFI mode for some particular reason? Do you think it will have any advantages?

Solved:
While the PC is in UEFI mode, the Windows PE version must match the PC architecture. A PC in 64-bit UEFI firmware mode can only boot 64-bit versions of Windows PE. A PC in 32-bit UEFI firmware mode can only boot 32-bit versions of Windows PE. On PCs that support both UEFI mode and legacy BIOS mode, you may be able to run 32-bit Windows PE on a 64-bit PC by changing BIOS menu settings from “UEFI mode” to “BIOS mode”, assuming the manufacturer supports legacy BIOS mode.

I also read in murphy78's Windows 10 x86 ISO that it has UEFI support for 32-bit processors, while Windows 10 x64 ISO has UEFI support for 64-bit processors.

On my 64-bit UEFI system, I can just either install a Windows x64 in UEFI or a Windows x86 in Legacy BIOS Mode.

Sorry, this is my first device with UEFI.

Use legacy mode to have a peaceful mind. What's the benefit of installing in UEFI mode? I don't think your computer will boot any faster or devices can be more compatible. It's up to drivers how devices behave.